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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
24/03/2021 |
Actualizado : |
14/06/2021 |
Tipo de producción científica : |
Artículos Indexados |
Autor : |
MARQUES, L. O. D.; MELO-FARIAS, P.; OLIVEIRA, R. P. DE; DINI, M.; SOARES FILHO, W. S.; MALGARIM, M. B. |
Afiliación : |
LÉO O. D. MARQUES, Departament of Horticulture and Crop Science, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil; PAULO MELLO-FARIAS, Departament of Horticulture and Crop Science, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil; ROBERTO P. DE OLIVEIRA, Embrapa Temperate Climate, Pelotas, Rio Grande do Sul, Brazil; MAXIMILIANO ANTONIO DINI VIÑOLY, Departament of Horticulture and Crop Science, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil; WALTER S. SOARES FILHO, Embrapa Cassava and Fruit Science, Cruz das Almas, Bahia, Brazil; MARCELO B. MALGARIM, Departament of Horticulture and Crop Science, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil. |
Título : |
Emergence percentage and speed of rootstocks for citriculture in south of Brazil. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Journal of Agricultural Science, 2019, v. 11, n. 5, p. 49-57. Doi: https://doi.org/10.5539/jas.v11n5p49 |
ISSN : |
1916-9752 E-ISSN 1916-9760 |
DOI : |
10.5539/jas.v11n5p49 |
Idioma : |
Inglés |
Notas : |
Article history: Received: January 21, 2019; Accepted: February 27, 2019; Online Published: April 15, 2019.
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brazil (CAPES)-Finance Code 001. |
Contenido : |
ABSTRACT.
The fact that Brazilian citriculture has focused on few rootstocks poses several phytosanitary risks to the culture and has made genetic improvement programs develop hybrid genotypes to be used as novel alternatives. This study aimed at evaluating the behavior of 42 different rootstocks regarding their emergence percentage and speed in weather conditions experienced in the extreme south of Brazil. Seeds of hybrids developed by the Citrus Genetic Improvement Program (PMG Citros) at the Embrapa Mandioca e Fruticultura (PMG Citros) and cultivars from other citrus growing regions were sown in conical tubes filled with commercial substrate in May 2017. A thoroughly randomized design with 4 replicates, each composed of 45 sampling units, was employed. Emergence percentage and emergence speed of every genotype were evaluated and the ones with the highest emergence percentage of seedlings and the ones with the most precocious emergence were identified. All genotypes completed seedling emergence 98 days after sowing. Seeds of Trifoliata, TSKC × CTSW-041 and TSKC × CTSW-025 had the highest values of seedling emergence whereas the highest emergence speed indexes were exhibited by genotypes Trifoliata and lemon tree 'Cravo'. |
Palabras claves : |
Adaptation; Diversification; Improvement; Seedlings. |
Thesagro : |
CITRUS; CITRUS spp. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15383/1/Marques-et-al.-2019.-Emergence-Percentage-and-Speed-of-Rootstocks-JAS.pdf
http://www.ccsenet.org/journal/index.php/jas/article/download/0/0/39038/39794.pdf
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Marc : |
LEADER 02347naa a2200289 a 4500 001 1061859 005 2021-06-14 008 2019 bl uuuu u00u1 u #d 022 $a1916-9752 E-ISSN 1916-9760 024 7 $a10.5539/jas.v11n5p49$2DOI 100 1 $aMARQUES, L. O. D. 245 $aEmergence percentage and speed of rootstocks for citriculture in south of Brazil.$h[electronic resource] 260 $c2019 500 $aArticle history: Received: January 21, 2019; Accepted: February 27, 2019; Online Published: April 15, 2019. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brazil (CAPES)-Finance Code 001. 520 $aABSTRACT. The fact that Brazilian citriculture has focused on few rootstocks poses several phytosanitary risks to the culture and has made genetic improvement programs develop hybrid genotypes to be used as novel alternatives. This study aimed at evaluating the behavior of 42 different rootstocks regarding their emergence percentage and speed in weather conditions experienced in the extreme south of Brazil. Seeds of hybrids developed by the Citrus Genetic Improvement Program (PMG Citros) at the Embrapa Mandioca e Fruticultura (PMG Citros) and cultivars from other citrus growing regions were sown in conical tubes filled with commercial substrate in May 2017. A thoroughly randomized design with 4 replicates, each composed of 45 sampling units, was employed. Emergence percentage and emergence speed of every genotype were evaluated and the ones with the highest emergence percentage of seedlings and the ones with the most precocious emergence were identified. All genotypes completed seedling emergence 98 days after sowing. Seeds of Trifoliata, TSKC × CTSW-041 and TSKC × CTSW-025 had the highest values of seedling emergence whereas the highest emergence speed indexes were exhibited by genotypes Trifoliata and lemon tree 'Cravo'. 650 $aCITRUS 650 $aCITRUS spp 653 $aAdaptation 653 $aDiversification 653 $aImprovement 653 $aSeedlings 700 1 $aMELO-FARIAS, P. 700 1 $aOLIVEIRA, R. P. DE 700 1 $aDINI, M. 700 1 $aSOARES FILHO, W. S. 700 1 $aMALGARIM, M. B. 773 $tJournal of Agricultural Science, 2019$gv. 11, n. 5, p. 49-57. Doi: https://doi.org/10.5539/jas.v11n5p49
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
22/12/2020 |
Actualizado : |
11/03/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
-- - -- |
Autor : |
CASTILLO, A.; LOPEZ, V.; TAVARES, E.; SANTIÑAQUE, F.; DALLA RIZZA, M. |
Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VICTOR JULIAN LOPEZ DEL PUERTO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. TAVARES, Forestal Oriental, Paysandú Uruguay.; F. SANTIÑAQUE, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Polyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs]. |
Complemento del título : |
Special Issue X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; XII Simposio REDBIO Argentina. Roca, William, Ed. 12 al 15 de noviembre de 2019, Montevideo, Uruguay. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Agrociencia Uruguay 2020, v. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413 |
Páginas : |
9 p. |
ISSN : |
2301-1548 |
DOI : |
10.31285/AGRO.24.381 |
Idioma : |
Inglés |
Notas : |
Article history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020). |
Contenido : |
Eucalyptus dunnii Maiden produces good quality cellulose pulp, showing good frost tolerance. However, in Uru-guay, it needs more than six years to reach the reproductive stage. Genome duplication was proposed as a strategy to obtain useful variability. The application of mitosis inhibitors for chromosomal duplication, in explants growing in vitro, was evaluated. Two antimitotic agents were used: colchicine and oryzalin in different concen-trations and exposure times, in two types of explants: explants growing in vitro and pre-germinated seeds. The number of chloroplasts was used as a rapid ploidy estimator and confirmed by flow cytometry. For chloroplast count, fluorescein diacetate (FDA) applied to in vitro leaves was used for staining. Oryzalin was effective for inducing plant duplication in E. dunnii from in vitro explants. In pre-germinated seeds, both antimitotic agents induced polyploids. The average number of chloroplasts was 5.5 in diploid control plants and more than 7 in tetraploids. Obtained plantlets were successfully cloned in the greenhouse. This is the first report on artificial polyploidy obtained in E. dunnii. |
Palabras claves : |
COLCHICINA; COLCHICINE; CULTIVO IN VITRO; EUCALIPTO; IN VITRO CULTURE; ORYZALIN; ORYZALINE; POLIPLOIDIA; POLYPLOIDY. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14914/1/Castillo-Agrociencia-2020.pdf
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Marc : |
LEADER 03227naa a2200325 a 4500 001 1061636 005 2021-03-11 008 2020 bl uuuu u00u1 u #d 022 $a2301-1548 024 7 $a10.31285/AGRO.24.381$2DOI 100 1 $aCASTILLO, A. 245 $aPolyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs].$h[electronic resource] 260 $c2020 300 $a9 p. 500 $aArticle history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020). 520 $aEucalyptus dunnii Maiden produces good quality cellulose pulp, showing good frost tolerance. However, in Uru-guay, it needs more than six years to reach the reproductive stage. Genome duplication was proposed as a strategy to obtain useful variability. The application of mitosis inhibitors for chromosomal duplication, in explants growing in vitro, was evaluated. Two antimitotic agents were used: colchicine and oryzalin in different concen-trations and exposure times, in two types of explants: explants growing in vitro and pre-germinated seeds. The number of chloroplasts was used as a rapid ploidy estimator and confirmed by flow cytometry. For chloroplast count, fluorescein diacetate (FDA) applied to in vitro leaves was used for staining. Oryzalin was effective for inducing plant duplication in E. dunnii from in vitro explants. In pre-germinated seeds, both antimitotic agents induced polyploids. The average number of chloroplasts was 5.5 in diploid control plants and more than 7 in tetraploids. Obtained plantlets were successfully cloned in the greenhouse. This is the first report on artificial polyploidy obtained in E. dunnii. 653 $aCOLCHICINA 653 $aCOLCHICINE 653 $aCULTIVO IN VITRO 653 $aEUCALIPTO 653 $aIN VITRO CULTURE 653 $aORYZALIN 653 $aORYZALINE 653 $aPOLIPLOIDIA 653 $aPOLYPLOIDY 700 1 $aLOPEZ, V. 700 1 $aTAVARES, E. 700 1 $aSANTIÑAQUE, F. 700 1 $aDALLA RIZZA, M. 773 $tAgrociencia Uruguay 2020$gv. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413
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